TLC - Thin Layer Chromatography

Thin Layer Chromatography (TLC) is used for isolation, identification and quantification of molecules from complex mixtures. Thin Layer Chromatography (TLC) is used for isolation, identification and quantification of molecules from complex mixtures.

It was developed in 20th century and it is still getting evolved with the advancement of technology

The technique has wide applications in Chemistry and Biology both being crucial for industrial application as well.

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Principle

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TLC works on the principle of difference in adsorption and capillary action of different molecules present within complex mixtures.

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Materials and Equipments

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Applications of TLC

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You can create your mini TLC experiment at home using coffee as the stationary phase & rubbing alcohol as the mobile phase. You can create your mini TLC experiment at home using coffee as the stationary phase & rubbing alcohol as the mobile phase. You can see the colors separate giving you a hands-on experience of chromatography! You can see the colors separate giving you a hands-on experience of chromatography!

Advantages of TLC

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TLC - Thin Layer Chromatography

TLC - Thin Layer Chromatography

The technique has wide applications in Chemistry and Biology both being crucial for industrial application as well.

The technique has wide applications in Chemistry and Biology both being crucial for industrial application as well.

Principle

Principle

TLC works on the principle of difference in adsorption and capillary action of different molecules present within complex mixtures.

TLC works on the principle of difference in adsorption and capillary action of different molecules present within complex mixtures.

Rapid Results

Materials and Equipments

– TLC plate – Developing chamber

– TLC plate – Developing chamber

– Developing chamber

– Developing chamber

– Sample

– Sample

– Solvent

– Solvent

– Spotting capillary or pipette – Visualisation tools or chemicals

– Spotting capillary or pipette – Visualisation tools or chemicals

Applications of TLC

– Pharmaceuticals – Material Sciences

– Pharmaceuticals – Material Sciences

– Forensic Science

– Forensic Science

– Life Sciences

– Life Sciences

– Environment Science – Food Industry

– Environment Science – Food Industry

Advantages of TLC

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