Antibiotics Classification based on mode of its action

Antibiotics

Antibiotics can be called as a savior or a superhero that fights with a villain. In this scenario, the villain is microscopic in size and they are not visible to us from naked eyes. They are omnivorous and we call them as bacteria. But not all the bacteria are bad for us.

Earlier a small scratch on body hold the potential to be fatal. The great influenza of 1918-19 killed tens of millions in an era without commercial planes. Until, the discovery of the first antibiotic called penicillin by Alexander Fleming from penicillium moulds in 1928.

Flemming accidentally left a dish of bacteria staphylococcus uncovered for a few days. When he returned, he found that the dish contains growth of mould. He observed that the bacterial growth was inhibited by the patch of mould.

The use of penicillin as a medicine was introduced by Howard Walter Florey, Ernest chain and Norman Heatley.

What are Antibiotics?

The word antibiotic means ‘against life’. The term was coined by Selman Waksman. When we expose the bacteria to antibiotics, it inhibit the bacterial multiplication (bacteriostatic) or kills it (bacteriocidal). Though they are superhero, but they some limitations too. Antibiotics cannot fight all the pathogenic villain. Antibiotic resistant bacteria and viruses cannot be inhibited by antibiotics. Hence, we need other strategy to fight with antibiotic resistant.

Who produces Antibiotics?

The antibiotics are produced by Bacteria and fungi. The antibiotic producing ability of such microbe is the part of their defense system. Antibiotic producing microbes uses antibiotics to attack on other microbes and increases the chance of their survival. But when we humans discovered antibiotics and its usage to fight the pathogenic bacteria, we started exploiting it.

Seeing the demand and need, these superheroes are produced on large scale in industries by the process of fermentation. In fermentation, the tank contains all the spell, flavours, strength, hate against bad bacteria which are called Growth medium which that provide the nutrition to the antibiotic producing microbes. After completion of antibiotic production, they are extracted and purified through multiple process and converted into crystal form. The Scientist have also attempted in synthesizing antibiotics artificially by chemical process.

Antibiotics classification based on mode of action

The antibiotic is a protein mostly produced by plazmid genes (extra chromosomal genes). These antibiotics may have different mechanism of inhibiting bacteria. They mostly attack on the vital molecular processes like bacterial cell wall synthesis, cell membrane, DNA replication, Transcription, Translation and metabolic pathways. The different authors classify antibiotics on different parameters. They can be classified on the basis of source of isolation, structure, spectrum and mode of action. The given articles explains the classification of antibiotics based on the mode of action.

I) Antibiotics that attacks on Cell Wall:

The Bacterial cell wall is made of peptidoglycan and the outer membrane is made up of lipopolysaccharide. The rigid structure of cell wall protects the cell from mechanical damage and maintains the osmotic balance. The most common antibiotics that attack on cell wall are penicllins, cephalosporins, bacitracin and vancomycin.

Penicillins:

Chemical nature/structure: It contains Beta lactum (Natural and Semisynthetic type) ring structure.

Source of isolation: Penicillum mold (Fungi).

Mode of action: Penicillin binds to cell wall synthesis proteins. Binding to such proteins inactivate them and interrupt the cell wall synthesis. The cell wall is made of polymer of alternate NAG and NAM molecule. These polymers are cross linked by 4 glycine molecules. The cross linking gives more rigidity to cell wall. The penicillin inhibits the crosslinking and making the cell wall fragile.

As the research was advanced,the natural penicillin G and V were structurally modified to increase the spectrum. This in-vitro modification brought new version of penicillin into the markets namely Amoxicillin, Ampicillin, Carbocypenicillin.

Cephalosporins:

Chemical nature/structure: It contains 4 membraned lactam ring, called beta lactum.

Source of isolation: Isolated from cultures of Acremonium strictum  (Fungi)

Mode of action: As it has beta lactum structure, the mode of action of Cephalosporins is similar to penicillin. Cephalosporin interferes with cell wall synthesis. The cell wall is made of heteropolymer of NAG and NAM. The crosslinking of these polymers are done by a protein called as Penicillin Binding Proteins PBP (because it has active site for penicilllin). Similar to penicillin, the Cephalosporins can also bind to the active site of PBP and causing its inhibition. The inhibited proteins cannot further carry out its function due to which the bacteria cannot synthesized the rigid cell wall. Cephalosporins are less susceptible to beta lactamase as compared to penicillins. Beta lactamase is an enzyme produced by beta lactum resistant antibiotics.

Bacitracin:

Chemical nature: It is a polypeptide with thiasoline ring.

Source of isolation: Bacillus subtilis

Mode of action: Bacitracin is able to inhibit or kill the bacteria based on its concentration. Low concentration acts as bacteriostatic (inhibit the bacterial growth) and high concentration acts as bacteriocidal (kills the bacteria). It binds to bacterial cell wall component C55-isoprenyl pyrophosphate and forms a complex and inhibit its function. This interferes with the cell wall formation.

Vancomycin

Chemical nature/structure: Glycopeptide

Source of isolation: Streptomyces orientalis

Mode of action: The Vancomycin hinder the cell wall synthesis by inhibiting transpeptidation. Loss of transpeptidation cause prevention of crosslinking and resulting in cell wall rigidity. Studies have also found that is binds and inhibits transglycosylase enzyme.

II) Cell membrane:

Cell membrane made of lipid bilayer, fencing the cytoplasm and internal organelles. It separates the internal environment from outside. It plays an important role in monitoring movement of molecules and ions across the membrane. Physical damage to cell membrane may leads to leakage of cellular components. The well known example of antibiotics that attack cell membrane are polymyxin B and polymyxin E (Colistin).

Chemical nature/structure: Lipopolypeptide

Source of isolation: Bacillus polymyxa

Mode of action: Polymyxin, a cationic polypeptide interferes with the ionic interaction of negatively charged lipopolysaccharide (LPS) of outer membrane of gram-negative bacteria with the positively charged associated proteins. It binds to the phosphate group of lipid A causing the displacement of magnesium and calcium ions. This disrupts the permeability and allowing the non-permeable molecules to enter the cell. The polymyxin B/E treated cells are more sensitive to penicillin and lysozyme.

III) Antibiotics that attacks on Protein synthesis:

Proteins are considered as one of the most vital biomolecule as it plays structural and functional role in maintaining the cell. The proteins are synthesized from genes by the process of transcription and translation. The antibiotics that attack protein synthesis machinery may inhibit ribosome or enzymes involved in protein synthesis. Inhibition of protein synthesis may lead to death of bacteria. The common protein inhibitors are Aminoglycosides, macrolides, lincosamides, streptogramins, chloramphenicol, tetracyclines.

1) Aminoglycosides:

Chemical nature: It has poly basic amino group liked to 2 or more amino sugar residues via glycosidic bond.

Source of isolation: Isolated from Streptomyces griseus (bacteria)

Mode of action: This antibiotic binds to 30s subunit of bacterial ribosome. They firstly penetrates the cell membrane through the aquas pours, they then reach to periplasmic place and then enters bacterial cell cytoplasm by active transport process, the antibiotic then targets the 30s subunit of ribosome and blocks the A site of 30s, Now the tRNA which is going to initiate the protein synthesis machinery by binding to A site, cannot initiate the process and ultimalety there will be no protein synthesis in the cytoplasm.

Examples: Streptomycin, Neomycin, Kanamycin, Gentamycin.

Macrolides

Chemical nature: It has macro-cyclic lactone ring with attached sugar, used in 1950- Erythromycin

Source of isolation: First used in 1952 and derived from Streptomyces erythreus (bacteria)

Mode of action: The macrolide inhibit the protein synthesis machinery by penetrating into the bacterial cell and  preventing peptidal translocation. It stops the adding of amino acids and eliminate the polymerisation of amino acids.

Example: Erythromycin(respiratory track), clarithromycin(pneumonia), azithromycin(infection of nose and throat), fidaxomicin(diarrhoea) and telithromycin(infection of lungs)

Streptogramins –

Chemical nature: Contains two groups (synergetic) :- depsipeptide (group I) and a lactonic macrocycle (group II). Quinupristin–dalfopristin is the only combination used in the clinic.

Mode of action:

It inhibits the protein synthesis machinery by binding to the 50s  subunit of ribosome. Quinupristin inhibit polypeptide elongation and induce early termination and forming incomplete and inactive protein. By binding of the molecule dalfopristin it induces a conformational change in the 50s subunit which helps in attachment of quinupristin more accurately.

Example: Lincosamides (clindamycin), oxazolidinones (linezolid, tedizolid)

Chloramphenicol

Chemical nature: It contains a nitro benzene ring, an amide bond and two alcohol functions.

Source of isolation: Streptomyces venezuelae

Mode of action: Acts on 50s subunit of ribosme and can also act on 70s subunit of mammalian cell. They act on 50 subunit by double mechanisms, they interfere with translation of the mRNA and inhibit the corporation of aminoacyl tRNA in the ribosome.

Tetracyclins

Chemical nature: It contains four, six member cyclic ring  and variety of functional groups are attached to it.

Source of isolation: It is derived from Streptomyces bacteria

Mode of action: These antibiotics attack the 30s subunit of bacterial ribosome and inhibit the synthesis of protein. These antibiotics are blocking the attachment of  new tRNA on to 30s subunit of ribosome at A site.

Example: Demeclocycline(respiratory track, skin ,acne, intestinal, genital), doxycycline(chest infection, rosacea, dental infection), minocycline (skin, eye, lymphatic, intestinal, genital, and urinary systems).

Antibiotics that attacks on DNA Replication:

DNA is a genetic material of most of the living cell. The genetic information is passed from one generation to another. The parental information is transferred to daugther cell is by making its copy. The process of making the replica of parental DNA is called Replication process. If Antibiotics attacks on DNA replication, the bacteria cannot multiply and eventually die. The antibiotics like quinolines, metronidazole, and rifampin attacks on DNA replication.

Quinolines-

Chemical nature: having bicyclic ring. There are two types of quinolines.

  1. Nalidixic acid: it has limited spectrum activity and have high frequence of bacterial resistance.
  2. Fluroquinolone: fluorination of quinolones at position 6, having wide spectrum activity.

Source of isolation: Synthetic

Mode of action:

Many enzymes are involved in synthesizing of proteins from the replication fork. For unwinding of DNA, it need DNA helicase. When DNA helicase unwinds the DNA, it causes topological strain (imagine you pulling 2 ropes which are tied around each other like DNA, and you unwinding it by pulling its two ends simultaneously). Topoisomerase is an enzyme which helps in solving this issue by cleaving strand. Now, this molecule of antibiotic interferes with the topoisomerase enzyme and it does not interfere with the activity of cleave but blocks the activity of ligation property of topoisomerase, and now they are behaving like endonuclease enzyme and now they cannot re-join the DNA. Ultimately the cell will Die, and this type of activity is known as bactericidal.

Examples-  ciprofloxacin (Cipro), lomefloxacin (Maxaquin), norfloxacin (Noroxin), ofloxacin (Floxin), moxifloxacin (Avelox) and levofloxacin (Levaquin).

Metronidazole

Mode of action– These type of antibiotic produce free superpower radicals and use to it fight against the bacteria. By using the oxidation-reduction potential they generate free radical which then damages the bacterial DNA.

Examples: Flagyl and Flagyl ER 

Rifampin

Drug category: Synthetic category of rifamycin having broad antibacterial activity, containing heterocyclic structure containing a naphthoquinone core spanned by an aliphatic chain.

Source of isolation: Streptomyces mediterranie (bacteria)

Mode of action: Rifampin penetrates the cell wall of bacteria and enters the cytosol and it stops the synthesis of mRNA, mRNA are having the anticodons at which the 30s subunit binds and start intatiting the tRNA and eventually peptide attaches to the tRNA one by one.And in this way it stops the synthesis of protein in bacterial cell.

Examples: Aprepitant, digoxin, praziquantel, ranolazine, sulfasalazine, tacrolimus, theophylline

These are used to treat tuberculosis.

Antibiotics that attacks on Metabolic Process:

The bacteria conducts different metabolic activity for synthesis and degradation of molecules. These vital metabolic activity are regulated by set of enzymes. The metabolic reaction also provides energy to the cell for conducting molecular and metabolic process. The  Sulfonamide and trimethoprim antibiotics kills the bacteria by inhibiting essential metabollic pathways.

Sulfonamide & Trimethoprim

Sulfonamides inhibit bacterial growth by suppressing nucleotide synthesis. The nucleotide syntheis occurs from tetrahydro folate. The tetrahydro folate is synthesised from Dihydrofolate. The Dihydrofolate is synthesized by dihydropteroate synthetase from p-aminobenzoic acid (PABA). The sulfonamide acts like competitive inhibitor. It binds to dihydropteroate synthetase in place of PABA. Due to the depletion of nucleotides, no DNA replication or synthesis occur resulting in stopping bacterial growth and multiplication.

Trimethoprim also inhibits nucleotide synthesis. It binds and inhibits dihydrofolate reductase (DHFR) which is involved in the synthesis of Tetrahydrofolate (THF).

Antibiotics inhibiting DNA replication
Mode of action of Sulfonamides and Trimethoprim

Hence, the mode of action of Sulfonalide and Trimethoprim is by suppressing nucleotide synthesis. The both the antibiotics acts like competitive inhibitor to the key enzymes and stops the synthesis at different step.

References:

https://www.sciencedirect.com/topics/neuroscience/bacitracin

http://tmedweb.tulane.edu/pharmwiki/doku.php/vancomycin

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5046685/

http://amrls.cvm.msu.edu/pharmacology/antimicrobials/antibiotics-of-veterinary-importance/sulfonamides

https://www.poultrymed.com/Sulfona

Saheb Singh

A Biotech student. Foodie, Traveller, Banging Tabla, smashing the Bhangra, a Paaji in Gujjiland.

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