Lab Protocol

“Plasmolysis term is derived from latin word plasma meaning matrix and lysis means loosening i.e. loosening of cell matrix”. Aim – To study and observe the plasmolysis phenomena of plant cells in the laboratory conditions. Theory – What is Plasmolysis? Plasmolysis is the process that describes the loss of water resulting in shrinkage of the

Staining technique is one of the fundamental technique in Microbiology used for observing the microbes under microscopes. The staining technique enhances and bring contrast in microbial specimen against the background making it visible under microscope. In staining, we use different staines and dyes to colour or highlight the specimen allowing us to explore microbial morphology.

Culture media is a nutrient rich liquid or gel the supports the growth of microorganisms. Microorganisms are omnipresent and grow in variety of habitat and needs varying nutrients. Therefore, we cannot expect or grow different microorganisms in the same media. Based on the requirements of microorganisms, the composition and concentration of nutrients varies. And hence,

“Bacterial colony morphology is the fundamental step for characterization and identification of bacteria“ In order to study microbes, we grow the bacteria in laboratory. We cultivate the bacteria in solid and liquid media. In solid media, the bacteria grow in the form of colonies. Colony is a dot-like structure that appears on the surface of

Light is an electromagnetic radiation. It is form of energy that travels in the form of waves. The distance between two consecutive crests is called as wavelength. The light spectrum has rays have different wavelengths. In the electromagnetic spectrum, at one end there is gamma rays and at the other end, there is radio waves.

Catalase and Oxidase tests are performed to check whether the given microorganism is aerobic or anaerobic. Oxidase Test – Oxidase test is performed to detect the presence of intracellular cytochrome oxidase system in the test organism. The cytochrome oxidase is component of Electron Transport Chain (ETC). Electron transport chain the last step of cellular respiration.