Lab Protocol

Phenol Coefficient Test

Determining the Phenol Coefficient of Test Disinfectant Phenol coefficient test is one of the methods used to estimate the disinfecting power of any disinfectant. Introduction to Phenol Coefficient Test: In phenol co-efficient method, we test the efficiency of chemical disinfectants.Disinfectant is an agent that inhibits or kills disease causing microorganisms. Disinfectant can be physical (heat […]

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Membrane Filtration of Heat Sensitive Material

Membrane Filtration of Heat Sensitive Material

Introduction In Microbiology Laboratory, the required material is sterilized either by dry or moist heat, but not every required material can be sterilized with heat. Therefore, the heat sensitive or labile materials are sterilized by membrane filtration method. When heat sensitive materials are exposed to heat, it leads to its denaturation.  For example, when Antibiotics

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Media Preparation | Easy Lab Protocol | BiokiMicroki

Media Preparation has become really simple these days. It is quite easy to make any complex media owing to the scientific advancements. Media Preparation Microorganisms are found everywhere; they are present in soil, water, air, and sewage and on the surface of animal and plant. Hence, they are called as omnivorous. As a microbiologist, if

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Indole Test | Easy Lab Protocol | BiokiMicroki

Introduction to Indole Test: Indole test is used for characterization of coliforms. Coliforms are the bacteria that are present in our digestive system. The coliforms are members of Enterobacteriaceae family. Morphologically these bacteria are rod shaped and gram-negative type. Indole test is one of the ideal methods used to distinguish the E.coli from Enterobacter and

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Thermal Darth

D and Z Values: Determination from a given bacterial culture

D and Z values are important terms to determine the efficiency of sterilization by using heat. Heat is one of the best physical methods for killing microbes because of its penetrating power. Heat causes dehydration and denaturation of cellular biomolecules. The tolerance of heat varies from microbe to microbe. The extent and rate of bacterial

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Biosafety Cabinet

Biosafety Cabinets: Principle and Application

Introduction to Biosafety Cabinets: Biosafety Cabinets are one of the most interesting safety equipments in a scientific lab. Often a part of sci-fi movies, these Biosafety Cabinets are enclosed workplace which are ventilated and provide protection to the user while handling contaminated or potentially contaminated micro-organisms. BSL Risk Diseases Treatment 1 No Does not cause

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Lab Hazards

Good Laboratory Practices- Must for all aspiring Scientists

An aspiring and budding Microbiologist should always initiate their study by first understanding and learning Good Laboratory Practices (GLP). These Practices are general rules and regulations that every Microbiologist should follow in the lab. We are aware that Experiments are always associated with risks, but in case of Microbiology Experiments, the level of risk is

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Demonstrating the presence of Microflora

Microflora: Demonstration Of its Presence in The Environment

Microflora is nowadays being considered as a misnomer as the term Microbiota is becoming more common in parlance. Introduction to Presence of Microflora : It is known fact that microorganisms are ubiquitous, they present in air, water, soil and surface of animate and inanimate objects. When microbes are present in air, they are in the

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ThisisEngineering RAEng

Bacterial Motility By Hanging Drop Techniques
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Bacterial Motility is an important characteristic which is used to identify an unknown micro-organisms. The Hanging Drop technique helps to determine if the bacteria is motile or not. Introduction to Bacterial Motility Motility of bacteria is it’s ability to move by its own at expense of energy. The bacteria either swim or glide in the aqueous

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