Introduction to TLC – Thin Layer Chromatography (TLC) is one of the chromatographic techniques used for isolation, identification and quantification of molecules from complex mixtures.
Category: Lab Protocol
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“Plasmolysis term is derived from latin word plasma meaning matrix and lysis means loosening i.e. loosening of cell matrix”. Aim – To study and observe
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Staining technique is one of the fundamental technique in Microbiology used for observing the microbes under microscopes. The staining technique enhances and bring contrast in
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Culture media is a nutrient rich liquid or gel the supports the growth of microorganisms. Microorganisms are omnipresent and grow in variety of habitat and
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“Bacterial colony morphology is the fundamental step for characterization and identification of bacteria“ In order to study microbes, we grow the bacteria in laboratory. We
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Dye Reduction tests are used to check the microbial load present in the milk sample. It is convenient and quick to use. AIM:- To carryout
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Yogurt is part of our diet from ancient time. It is been produced and consumed from thousand of years. It is obtained from milk. The
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Introduction Winogradsky’s column is used to study cross section of a natural environment in the laboratory. Sergei Winogradsky invented the column in the 1880s. Various
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Light is an electromagnetic radiation. It is form of energy that travels in the form of waves. The distance between two consecutive crests is called
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Catalase and Oxidase tests are performed to check whether the given microorganism is aerobic or anaerobic. Oxidase Test – Oxidase test is performed to detect
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Catalase and Oxidase tests are performed to check whether the given microorganism is aerobic or anaerobic. Catalase Test – The catalase enzyme protects the cell
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pH indicates the concentration of hydrogen ions (H+) of any solution. The Hydrogen ions are protons hence, it is also the measurement of protons in
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Introduction – Bacterial Growth is affected by nutritional and physical factors. The nutrients means the organic and inorganic material whereas the physical factors means pH,
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Introduction- While working in lab, we need to prepare different solutions of different concentration. Dilution is defined as the process of decreasing the concentration of
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Introduction to Acid Fast Staining- In 1882, Paul Ehrlich developed the Acid Fast Staining technique. Later, Ziehl and Neelson developed it in 1883 and hence